Children |
| 1. Prince of Portugal Affonso, b. 24 Feb 1526, Almeria, Almeria, Portugal |
| 2. Princess of Portugal Maria, b. 15 Oct 1527, Coimbra, Coimbra, Portugal |
| 3. Princess of Portugal Izabel, b. 28 Apr 1529, Lisboa, Lisboa, Portugal |
| 4. Princess of Portugal Brites, b. 15 Feb 1530, Lisboa, Lisboa, Portugal |
| 5. Prince of Portugal Manuel, b. 1 Nov 1531, Alvito, Beja, Portugal |
| 6. Prince of Portugal Filipe, b. 25 May 1533, Évora, Evora, Portugal |
| 7. Prince of Portugal Diniz, b. 26 Apr 1535, Évora, Evora, Portugal |
| 8. Prince of Portugal And The Algarves Jodao, b. 3 Jun 1537, Évora, Evora, Portugal |
| 9. Prince of Portugal Antonio, b. 9 Mar 1539, Lisboa, Lisboa, Portugal |
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